A novel method for isolation of large insert DNA from recombinant lambda DNA

A Novel Large-Scale Deletion of The Mitochondrial DNA of Spermatozoa of Men in North Iran

evaluation of a modified dna extraction method for isolation of cell-free fetal dna from maternal serum

background: discovery of short cell free fetal dna (cffdna) fragments in maternal plasma has created major changes in the field of prenatal diagnosis. the use of cffdna to set up noninvasive prenatal test is limited due to the low concentration of fetal dna in maternal plasma therefore, employing a high efficiency extraction method leads to more accurate results. the aim of this study was to ev...

A REPORT OF RAPID AND EFFICIENT METHOD FOR MITOCHONDRIAL DNA

Based on the similarity of prokaryotic DNA to that of mitochondrial DNA, a rapid and efficient method for DNA purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by Marmur (1983) which resulted in a high degree of purity (A260/A280=2). In addition we have applied the same technique for the first time to purify...

Rapid preparation and identification of insert-containing recombinant plasmid DNA.

The identification of transformant bacterial colonies containing potential recombinant DNA clones can be performed using a wide variety of screening methods including colony hybridization and autoradiography, polymerase chain reaction (PCR) and restriction endonuclease analysis of individual plasmid DNA (1,5). While colony hybridization can efficiently screen large numbers of recombinant clones...

An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA

  Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...